IDO2 Polyclonal antibody

IDO2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30530-1-AP

Synonyms

IDO 2, IDO2, INDOL1, indoleamine 2,3 dioxygenase 2

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Validation Data Gallery

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  WB analysis using 30530-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30530-1-AP (IDO2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30530-1-AP targets IDO2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDO2 fusion protein Ag33013
• Full Name: indoleamine 2,3-dioxygenase 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: NM_194294
• Gene Symbol: IDO2
• Gene ID (NCBI): 169355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDO2 is one of two closely related tryptophan catabolizing enzymes induced under inflammatory conditions. In contrast to the immunoregulatory role defined for IDO1 in cancer models, IDO2 has a proinflammatory function in models of autoimmunity and contact hypersensitivity. The product of the IDO2 gene is a 420 amino-acid protein, with a molecular weight of ~47 kDa. (PMID: 34965962, PMID: 29807576, PMID: 35493480)