IDO1 Recombinant antibody

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  WB analysis of HeLa using 84063-1-RR

  IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 84063-1-RR (IDO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84063-1-RR

  1x10^6 IFN-gamma treated HeLa cells were intracellularly stained with 0.25 ug Ido1 Recombinant Antibody (84063-1-RR, Clone:241110C8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84063-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84063-1-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

IDO1 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84063-1-RR

Clone No.241110C8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

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Tested Applications

• Positive WB detected in: IFN gamma treated HeLa cells
• Positive FC (Intra) detected in: IFN-gamma treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84063-1-RR targets IDO1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IDO1 fusion protein Ag3953
• Full Name: indoleamine 2,3-dioxygenase 1
• Calculated Molecular Weight: 403 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC027882
• Gene Symbol: IDO1
• Gene ID (NCBI): 3620
• RRID: AB_3671632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14902
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).