IDO1 Monoclonal antibody, PBS Only

IDO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G2G11

Cat no : 66528-1-PBS

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

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Validation Data Gallery

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  WB analysis using 66528-1-Ig (same clone as 66528-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66528-1-Ig (same clone as 66528-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 66528-1-Ig (same clone as 66528-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66528-1-Ig (same clone as 66528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66528-1-Ig (IDO1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 66528-1-Ig (same clone as 66528-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using IDO1 antibody (66528-1-Ig, Clone: 3G2G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

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Product Information

66528-1-PBS targets IDO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IDO1 fusion protein Ag3953
• Full Name: indoleamine 2,3-dioxygenase 1
• Calculated Molecular Weight: 403 aa, 45 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC027882
• Gene Symbol: IDO1
• Gene ID (NCBI): 3620
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).