IDI1 Polyclonal antibody, PBS Only

IDI1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11166-2-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11166-2-AP (same clone as 11166-2-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 11166-2-AP (same clone as 11166-2-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11166-2-AP (IDI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 11166-2-AP (same clone as 11166-2-PBS)

  IP result of anti-IDI1 (IP:11166-2-AP, 4ug; Detection:11166-2-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11166-2-AP (same clone as 11166-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11166-2-AP (IDI1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11166-2-PBS in a different storage buffer formulation.

---

Product Information

11166-2-PBS targets IDI1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDI1 fusion protein Ag1636
• Full Name: isopentenyl-diphosphate delta isomerase 1
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC019227
• Gene Symbol: IDI1
• Gene ID (NCBI): 3422
• RRID: AB_2233532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IDI1(Isopentenyl-diphosphate Delta-isomerase 1) is a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP). It belongs to the IPP isomerase type 1 family. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 26 kDa and 32 kDa.