IDH3G Polyclonal antibody

IDH3G Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25848-1-AP

Synonyms

NAD(+)-specific ICDH subunit gamma, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H IDHG

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Validation Data Gallery

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  WB analysis of mouse colon using 25848-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25848-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 25848-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 25848-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse colon tissue, A375 cells, A431 cells
• Positive IHC detected in: human colon tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25848-1-AP targets IDH3G in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDH3G fusion protein Ag23113
• Full Name: isocitrate dehydrogenase 3 (NAD+) gamma
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC000933
• Gene Symbol: IDH3G
• Gene ID (NCBI): 3421
• RRID: AB_2880267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncogene; Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.; Authors - Ke Tang