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IDH3B Monoclonal antibody, PBS Only

IDH3B Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11A9

Cat no : 68199-1-PBS

Synonyms

FLJ11043, H IDHB, IDH3B, RP46

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH3B Monoclonal antibody (68199-1-Ig)

    WB analysis using 68199-1-Ig (same clone as 68199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68199-1-Ig (IDH3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68199-1-PBS in a different storage buffer formulation.

Product Information

68199-1-PBS targets IDH3B in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IDH3B fusion protein Ag8693
Full Name isocitrate dehydrogenase 3 (NAD+) beta
Calculated Molecular Weight 42 kDa
Observed Molecular Weight39-42 kDa
GenBank Accession NumberBC001960
Gene Symbol IDH3B
Gene ID (NCBI) 3420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).