IDH2 Recombinant antibody, PBS Only

IDH2 Uni-rAb^TM Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1

Cat no : 82117-1-PBS

Synonyms

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase

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Validation Data Gallery

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  WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 82117-1-RR (same clone as 82117-1-PBS)

  IP result of anti-IDH2 (IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 82117-1-RR (same clone as 82117-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

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Product Information

82117-1-PBS targets IDH2 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IDH2 fusion protein Ag8779
• Full Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC009244
• Gene Symbol: IDH2
• Gene ID (NCBI): 3418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.