IDH2 Polyclonal antibody, PBS Only

IDH2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23254-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 23254-1-AP (same clone as 23254-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of DU 145 using 23254-1-AP (same clone as 23254-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 23254-1-AP (same clone as 23254-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 23254-1-AP (same clone as 23254-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23254-1-AP (same clone as 23254-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 23254-1-AP (same clone as 23254-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 23254-1-AP (same clone as 23254-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23254-1-AP (IDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

---

Product Information

23254-1-PBS targets IDH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDH2 fusion protein Ag19748
• Full Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 41-47 kDa
• GenBank Accession Number: BC009244
• Gene Symbol: IDH2
• Gene ID (NCBI): 3418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved. This antibody is specific to IDH2.