IDE Monoclonal antibody

• Featured Product
• KD/KO Validated

IDE Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

3E10G1

Cat no : 67106-1-Ig

Synonyms

Abeta degrading protease, FLJ35968, IDE, INSULYSIN

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Validation Data Gallery

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  WB analysis using 67106-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67106-1-Ig

  WB result of IDE antibody (67106-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells.

• 

  WB analysis of NIH/3T3 using 67106-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67106-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67106-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67106-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 67106-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67106-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67106-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67106-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67106-1-Ig (IDE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, A431 cells, HEK-293 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below

Product Information

67106-1-Ig targets IDE in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IDE fusion protein Ag17161
• Full Name: Abeta degrading protease
• Calculated Molecular Weight: 1019 aa, 118 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC096336
• Gene Symbol: IDE
• Gene ID (NCBI): 3416
• RRID: AB_2882410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).

Publications

Species	Application	Title
human,mouse	WB	Aging Cell; Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease; Authors - Guoxin Zhang
mouse	WB	Mol Nutr Food Res; Long-Term High-Fat Diet Decreases Renal Insulin-Degrading Enzyme Expression and Function by Inhibiting the PPARγ Pathway; Authors - Qian Su
mouse	WB	Mol Med; Inhibition of insulin degrading enzyme suppresses osteoclast hyperactivity via enhancing Nrf2-dependent antioxidant response in glucocorticoid-induced osteonecrosis of the femoral head; Authors - Tao Yuan
mouse	WB	Phytomedicine; Osmundacetone ameliorates Alzheimer's-like pathologies by inhibiting β-amyloid fibrillation, oxidative damage and neuroinflammation in APP/PS1 transgenic mice; Authors - Ling-Xiao Zhao
human	WB	Phytomedicine; Jiawei Xionggui Decoction promotes meningeal lymphatic vessels clearance of β-amyloid by inhibiting arachidonic acid pathway; Authors - Xiao Liu