IDE Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IDE Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21728-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 21728-1-AP (same clone as 21728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21728-1-AP (same clone as 21728-1-PBS)

  WB result of IDE antibody (21728-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDE transfected HeLa cells. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A2780 using 21728-1-AP (same clone as 21728-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 21728-1-AP (IDE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 21728-1-AP (same clone as 21728-1-PBS)

  IP result of anti-IDE (IP:21728-1-AP, 4ug; Detection:21728-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 21728-1-AP (same clone as 21728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 21728-1-AP (IDE antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21728-1-PBS in a different storage buffer formulation.

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Product Information

21728-1-PBS targets IDE in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDE fusion protein Ag16392
• Full Name: Abeta degrading protease
• Calculated Molecular Weight: 1019 aa, 118 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC096336
• Gene Symbol: IDE
• Gene ID (NCBI): 3416
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IDE (INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531). This antibody is specific to IDE.