ICOSLG/B7-H2/CD275 Monoclonal antibody

ICOSLG/B7-H2/CD275 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G1F8

Cat no : 67003-1-Ig

Synonyms

B7H2, ICOSLG, B7RP1, B7-related protein 1, B7-like protein Gl50

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Validation Data Gallery

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  WB analysis using 67003-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 67003-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, Daudi cells, HUVEC cells, JAR cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67003-1-Ig targets ICOSLG/B7-H2/CD275 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ICOSLG/B7-H2/CD275 fusion protein Ag6799
• Full Name: inducible T-cell co-stimulator ligand
• Calculated Molecular Weight: 33 kDa, 34 kDa
• Observed Molecular Weight: 60-65 kDa, 28-35 kDa
• GenBank Accession Number: BC064637
• Gene Symbol: ICOSLG
• Gene ID (NCBI): 23308
• RRID: AB_2882320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).