ICOSLG/B7-H2/CD275 Polyclonal antibody

ICOSLG/B7-H2/CD275 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14922-1-AP

Synonyms

B7H2, ICOSLG, B7 H2, B7RP-1, B7RP1

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Validation Data Gallery

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  WB analysis using 14922-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14922-1-AP (ICOSLG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14922-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14922-1-AP (ICOSLG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14922-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14922-1-AP (ICOSLG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells, JAR cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

14922-1-AP targets ICOSLG/B7-H2/CD275 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ICOSLG/B7-H2/CD275 fusion protein Ag6392
• Full Name: inducible T-cell co-stimulator ligand
• Calculated Molecular Weight: 33 kDa, 34 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC064637
• Gene Symbol: ICOSLG
• Gene ID (NCBI): 23308
• RRID: AB_2122732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.; Authors - Ha-Yeon Shin
human	IHC	Cell Mol Gastroenterol Hepatol; Sex Differences in Genomic Features of Hepatitis B-Associated Hepatocellular Carcinoma With Distinct Antitumor Immunity; Authors - Chungui Xu