ICOS Polyclonal antibody

ICOS Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 13338-1-AP

Synonyms

Inducible T cell costimulator, inducible T cell co stimulator, CVID1, CD278, AILIM

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Validation Data Gallery

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  WB analysis of Jurkat using 13338-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 13338-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 13338-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13338-1-AP (ICOS antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 13338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using ICOS antibody (13338-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HL-60 cells
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13338-1-AP targets ICOS in WB, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ICOS fusion protein Ag4155
• Full Name: inducible T-cell co-stimulator
• Calculated Molecular Weight: 199 aa, 23 kDa
• Observed Molecular Weight: 28 kDa, 45-50 kDa
• GenBank Accession Number: BC028210
• Gene Symbol: ICOS
• Gene ID (NCBI): 29851
• RRID: AB_2122590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13. Catalog#13338-1-AP recognizes two bands of 25-30 kDa and 45-50 kDa, and the additional 45-50 kDa band may be due to  surface disulfide-linked homodimeric glycoprotein.