ICAM4 Monoclonal antibody, PBS Only

ICAM4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A9B9

Cat no : 67014-1-PBS

Synonyms

CD242, ICAM 4, ICAM4, ICAM-4, LW, LW blood group protein

---

Validation Data Gallery

• 

  WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

  Different amount of human blood lysates were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human red blood cells using 67014-1-Ig (same clone as 67014-1-PBS)

  human red blood cells were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

---

Product Information

67014-1-PBS targets ICAM4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ICAM4 fusion protein Ag27628
• Full Name: intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
• Calculated Molecular Weight: 271 aa, 29 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC029364
• Gene Symbol: ICAM4
• Gene ID (NCBI): 3386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.