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  • Western Blot (WB) analysis of various lysates using ICAM3/CD50 Recombinant antibody (84335-5-RR)

    WB analysis using 84335-5-RR (same clone as 84335-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84335-5-RR (ICAM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84335-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84335-5-RR

    Affinity and Kinetic Characterization of 84335-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84335-5-RR against Human ICAM3/CD50 were performed. The affinity constant is 24.7 nM.

ICAM3/CD50 Recombinant antibody, PBS Only

ICAM3/CD50 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84335-5-PBS
Clone No.241417F3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CDW50, ICAM3, ICAM R, ICAM 3, CD50

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

84335-5-PBS targets ICAM3/CD50 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein Predict reactive species
Full Name intercellular adhesion molecule 3
Calculated Molecular Weight 547 aa, 60 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC058903
Gene Symbol ICAM3/CD50
Gene ID (NCBI) 3385
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32942
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Intercellular Adhesion Molecule 3 (ICAM-3), also known as CD50, is a member of the immunoglobulin superfamily. It is a transmembrane glycoprotein that contains an N-terminus extracellular domain, a single transmembrane domain, and a C-terminus cytoplasmic domain. ICAM-3 possesses 2-9 Ig-like C2-type domains in its extracellular region and is known to bind to the leukocyte adhesion protein LFA-1 . This molecule is expressed on leukocytes and epidermal Langerhans cells and plays a significant role in immune cell interactions and T lymphocyte activation.