ICAM2/CD102 Monoclonal antibody

ICAM2/CD102 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H3C6

Cat no : 67628-1-Ig

Synonyms

CD102, ICAM2, ICAM-2, ICAM 2, 1H3C6

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Validation Data Gallery

• 

  WB analysis of human peripheral blood platelets using 67628-1-Ig

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67628-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67628-1-Ig (ICAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HUVEC using 67628-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ICAM2/CD102 antibody (67628-1-Ig, Clone: 1H3C6 ) at dilution of 1:1991 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: human peripheral blood platelets, Jurkat cells
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:995-1:3982

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67628-1-Ig targets ICAM2/CD102 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ICAM2/CD102 fusion protein Ag29441
• Full Name: intercellular adhesion molecule 2
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC003097
• Gene Symbol: ICAM2/CD102
• Gene ID (NCBI): 3384
• RRID: AB_2882829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.