Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98049-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (98049-1-RR, Clone:240249D10) or 0.25 ug Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD3. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98049-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse ICAM-1/CD54 Rabbit Recombinant Antibody (98049-1-RR, Clone:240249D10)(red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98049-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98049-1-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.49 nM.

ICAM-1/CD54 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98049-1-RR

Clone No.240249D10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD54, Icam-1, MALA-2, Icam1, ICAM 1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98049-1-RR targets ICAM-1/CD54 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• RRID: AB_3672196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P13597
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).