Mouse ICAM-1/CD54 Recombinant antibody, PBS Only

ICAM-1/CD54 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E23

Cat no : 82827-1-PBS

Synonyms

CD54, ICAM-1, MALA-2, Icam1, ICAM 1

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Validation Data Gallery

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  WB analysis using 82827-1-RR (same clone as 82827-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82827-1-RR (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 82827-1-RR (same clone as 82827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

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Product Information

82827-1-PBS targets ICAM-1/CD54 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 85-110 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).