ICAM-1/CD54 Polyclonal antibody

ICAM-1/CD54 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 16174-1-AP

Synonyms

ICAM-1, CD54, MALA-2, Icam1, ICAM 1

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Validation Data Gallery

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  WB analysis using 16174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16174-1-AP (ICAM-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 16174-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16174-1-AP (Icam-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 16174-1-AP

  IP result of anti-ICAM-1 (IP:16174-1-AP, 4ug; Detection:16174-1-AP 1:3000) with mouse spleen tissue lysate 1920 ug.

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  IHC staining of mouse lung using 16174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16174-1-AP (ICAM-1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 16174-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16174-1-AP (ICAM-1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of 4T1 using 16174-1-AP

  Immunofluorescent analysis 4T1 cells using 16174-1-AP (Icam-1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of 4T1 using 16174-1-AP

  Immunofluorescent analysis of 4T1 cells using 16174-1-AP (Icam-1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

16174-1-AP targets ICAM-1/CD54 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: NM_010493
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 15894
• RRID: AB_2248702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).

Publications

Species	Application	Title
human,mouse	WB	Adv Sci (Weinh); Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury; Authors - Xun Wu
mouse,rat	WB	Clin Sci (Lond); Cardioprotective and anti-inflammatory effects of Caveolin 1 in experimental diabetic cardiomyopathy; Authors - Wenyan Gong
human,mouse	WB,IF	J Neuroinflammation; SARS-CoV-2 productively infects human brain microvascular endothelial cells.; Authors - Rui-Cheng Yang
human	WB	J Clin Endocrinol Metab; LncRNA LPAL2/miR-1287-5p/EGFR axis modulates TED derived orbital fibroblast activation through cell adhesion factors.; Authors - Nuo Wang
rat	IHC	Transplantation; The Selective RNA Polymerase I Inhibitor CX-5461 Mitigates Neointimal Remodeling in a Modified Model of Rat Aortic Transplantation.; Authors - Chaochao Dai
mouse	WB	Front Pharmacol; Precise Investigation of the Efficacy of Multicomponent Drugs Against Pneumonia Infected With Influenza Virus.; Authors - Junying Wei