human IBA1 Monoclonal antibody, PBS Only

human IBA1 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6A10

Cat no : 66827-1-PBS

Synonyms

AIF1, IBA1, G1, allograft inflammatory factor 1, AIF-1

---

Validation Data Gallery

• 

  IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

---

Product Information

66827-1-PBS targets human IBA1 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: human IBA1 fusion protein Ag28236
• Full Name: allograft inflammatory factor 1
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: BC009474
• Gene Symbol: IBA1
• Gene ID (NCBI): 199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.