Filter: AllIHC

IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

human IBA1 Monoclonal antibody, PBS Only

human IBA1 Monoclonal Antibody for IHC, ELISA

Cat No. 66827-1-PBS

Clone No.1C6A10

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Synonyms

AIF1, IBA1, 1C6A10, AIF 1, AIF-1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66827-1-PBS targets human IBA1 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	human IBA1 fusion protein Ag28236 Predict reactive species
Full Name	allograft inflammatory factor 1
Calculated Molecular Weight	17 kDa
GenBank Accession Number	BC009474
Gene Symbol	IBA1
Gene ID (NCBI)	199
ENSEMBL Gene ID	ENSG00000204472
RRID	AB_2882170
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P55008
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.