IBA1 Monoclonal antibody, PBS Only

IBA1 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6A10

Cat no : 66827-1-PBS

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

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Validation Data Gallery

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  IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66827-1-Ig (same clone as 66827-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-1-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 66827-1-Ig (same clone as 66827-1-PBS)

  1X10^6 THP-1 cells were intracellularly stained with 0.2 ug Anti-Human IBA1 (66827-1-Ig, Clone:1C6A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66827-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66827-1-PBS targets IBA1 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IBA1 fusion protein Ag28236
• Full Name: allograft inflammatory factor 1
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: BC009474
• Gene Symbol: AIF1
• Gene ID (NCBI): 199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.