Human YAP1 ELISA Kit
Cat no : KE00308
Synonyms
65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog
Validation Data Gallery
Product Information
KE00308 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human YAP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human YAP1. The assay recognizes human YAP1. An antibody specific for human YAP1 has been pre-coated onto the microwells. The human YAP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human YAP1 is added to detect the captured human YAP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human YAP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10413 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 90% | 81%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,582.1 | 46.9 | 1.8 |
| 2 | 20 | 604.5 | 21.3 | 3.5 |
| 3 | 20 | 140.6 | 3.9 | 2.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,728.1 | 89.3 | 3.3 |
| 2 | 24 | 605.1 | 28.8 | 4.8 |
| 3 | 24 | 136.4 | 7.2 | 5.3 |
Background Information
YAP1 is a transcriptional regulator and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog |