Human VE-cadherin/CD144 ELISA Kit
Cat no : KE00556
Synonyms
VE-cadherin, 7B4, 7B4 antigen, Cadherin 5, CD144
Validation Data Gallery
Product Information
KE00556 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human VE-cadherin/CD144 ELISA kit is to be used to detect and quantify protein levels of endogenous human VE-cadherin/CD144. The assay recognizes human VE-cadherin/CD144. An antibody specific for human VE-cadherin/CD144 has been pre-coated onto the microwells. The human VE-cadherin/CD144 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human VE-cadherin/CD144 is added to detect the captured human VE-cadherin/CD144 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human VE-cadherin/CD144 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1003 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 100% | 96%-105% |
| Cell culture supernatant | 102% | 95%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 10.60 | 0.51 | 4.85 |
| 2 | 8 | 3.09 | 0.08 | 2.59 |
| 3 | 8 | 2.06 | 0.02 | 0.89 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.05 | 0.78 | 7.07 |
| 2 | 16 | 3.12 | 0.16 | 5.03 |
| 3 | 16 | 0.87 | 0.03 | 3.94 |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins. VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium. VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium. VE-cadherin has also been shown to be required for angiogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | VE-cadherin, 7B4, 7B4 antigen, Cadherin 5, CD144 |