Human Total MMP-3 ELISA Kit
Cat no : KE00160
Synonyms
CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, MMP-3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1
Validation Data Gallery
Product Information
KE00160 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total MMP3 ELISA kit is to be used to detect and quantify protein levels of endogenous Total MMP3. The assay recognizes human Total MMP3. An antibody specific for Total MMP3 has been pre-coated onto the microwells. The Total MMP3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Total MMP3 is added to detect the captured Total MMP3 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total MMP-3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 18.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4314 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 89%-103% |
| Cell culture supernatants | 96% | 78%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 60.6 | 5.2 | 8.6 |
| 2 | 20 | 245.4 | 20.4 | 8.3 |
| 3 | 20 | 954.7 | 43.7 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 57.8 | 5.3 | 9.2 |
| 2 | 24 | 201.8 | 12.5 | 6.2 |
| 3 | 24 | 870.9 | 57.0 | 6.5 |
Background Information
MMP-3 (matrix metallopeptidase 3), also named as Stromelysin 1 or STR1, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis and tissue remodelling and in many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fbronectin, laminin, IGFBP-3, serpins, and IL-1β.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, MMP-3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Gut Microbes Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway | ||
Aging Cell Activation of epidermal growth factor receptor signaling mediates cellular senescence induced by certain pro-inflammatory cytokines. | ||
EBioMedicine Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis | ||
Int Immunopharmacol CX-4945 inhibits fibroblast-like synoviocytes functions through the CK2-p53 axis to reduce rheumatoid arthritis disease severity | ||
ACS Omega Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells |