Human Total MMP-1 ELISA Kit
Cat no : KE00223
Synonyms
CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1
Validation Data Gallery
Product Information
KE00223 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-1. The assay recognizes human MMP-1. An antibody specific for MMP-1 has been pre-coated onto the microwells. The MMP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human MMP-1 is added to detect the captured human MMP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total MMP-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 7.7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4312 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 81%-108% |
| Cell culture supernatants | 85% | 77%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,037.2 | 28.6 | 2.8 |
| 2 | 20 | 244.1 | 5.6 | 2.3 |
| 3 | 20 | 58.3 | 1.9 | 3.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,050.6 | 23.5 | 2.2 |
| 2 | 24 | 244.8 | 6.9 | 2.8 |
| 3 | 24 | 57.3 | 2.9 | 5.1 |
Background Information
Matrix metalloproteinase 1 (MMP1), which is also known as interstitial collagenase and fibroblast collagenase is a member of the matrix metalloproteinases (MMPs) family . Proteins in this family mainly participate in the breakdown of extracellular matrix both in normal physiological processes and disease processes. Several factors contribute to the expression of MMP1, including endogenous factors, such as polymorphisms and epigenetic regulation in the promoter region of MMP1, as well as exogenous factors, such as the tumor microenvironment. A variety of MMP1 promoter genotypic polymorphisms have been reported in various ethnic populations, and their contributions to different disease risks have been explored. In the tumor microenvironment, a variety of inflammatory factors, including interleukin-8 (IL-8), IL-1β and tumor necrosis factor-α (TNF-α), have been reported to induce MMP1 expression in cancer cells. (PMID: 31115512, PMID: 28334049)
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Omega Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells | ||
Biomolecules Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies |