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Recombinant Human TNFR1/CD120a protein (His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 0.8-3.2 ng/mL

Cat no : Eg0531

Validation Data Gallery

  • Purity of Recombinant Human TNFR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TNFR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.

    Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.
Expression HEK293-derived Human TNFR1 protein Ile22-Thr211 (Accession# P19438-1) with a His tag at the C-terminus.
GeneID 7132
Accession P19438-1
PredictedSize 22.1 kDa
SDS-PAGE 32-48 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55 kDa TNFR1. Soluble TNFR1 (sTNFR1) could function as TNF-binding protein, inhibiting TNF bioactivity.

References:

1.  Islam A, et al. (2006). J Biol Chem. 281(10):6860-73.
2.  Aggarwal BB, et al. (2012). Blood. 119(3):651-65.
3.  Aderka D , et al. (2012). J Exp Med. 175(2):323-9.