AuthentiKine™ Human TNF-alpha ELISA Kit


8.6 pg/mL


15.6-500 pg/mL



Cat no : KE00154


Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

Product Information

KE00154 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNF-alpha ELISA kit is to be used to detect and quantify protein levels of endogenous TNF-alpha. The assay recognizes human TNF-alpha. An antibody specific for TNF-alpha has been pre-coated onto the microwells. The TNF-alpha protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TNF-alpha is added to detect the captured TNF-alpha protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human TNF-alpha ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 8.6 pg/mL
Range 15.6-500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 7124


Sample TypeAverageRange
Human Plasma10282-117
Cell culture supernatants9585-106


Samplenmean ( pg/mL)SDCV%


Samplenmean ( pg/mL)SDCV%

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Dysregulation of TNF production has been implicated in a variety of human diseases including Alzheimer's disease, cancer, major depression and inflammatory bowel disease (IBD).


Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor


SpeciesSample TypeTitle

J Immunother Cancer

All-trans retinoic acid improves NSD2-mediated RARα phase separation and efficacy of anti-CD38 CAR T-cell therapy in multiple myeloma

Authors - Ziyi Peng
humancell lysates

Oxid Med Cell Longev

Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.

Authors - Xin Li
humancell culture supernatants

Oxid Med Cell Longev

Catalpol-Induced AMPK Activation Alleviates Cisplatin-Induced Nephrotoxicity through the Mitochondrial-Dependent Pathway without Compromising Its Anticancer Properties.

Authors - Jiangnan Zhang
humancell culture supernatants

Oxid Med Cell Longev

PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.

Authors - Zeyu Li
humancell culture supernatants

Int J Mol Sci

Abiotrophia defectiva DnaK Promotes Fibronectin-Mediated Adherence to HUVECs and Induces a Proinflammatory Response.

Authors - Minoru Sasaki
humancell culture supernatants


Role of inflammation in the malignant transformation of pleural mesothelial cells induced by multi-walled carbon nanotubes.

Authors - Xiaopei Huang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Trim (Verified Customer) (12-04-2020)

Nice sensitivity of the kit.

  • Applications: Other
  • Primary Antibody Dilution: Not diluted for serum.
  • Cell Tissue Type: Serum