Human T Cell Basics Panel

Host/Isotype

Mouse/Various

Reactivity

Human

Applications

FC

Conjugate

Various

Cat no : PK30007

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Validation Data Gallery

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  1x10^6 Human PBMCs were stained with PK30007 Human T Cell Basics Panel. CD45+ cells are gated. Parent population: lymphocytes. Cells were not fixed.

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  1x10^6 Human PBMCs were stained with PK30007 Human T Cell Basics Panel. CD3+/CD19- cells are gated. Parent population: CD45+ lymphocytes. Cells were not fixed.

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  1x10^6 Human PBMCs were stained with PK30007 Human T Cell Basics Panel. CD4 and CD8 expression on CD45+CD3+CD19- lymphocytes are shown. Cells were not fixed.

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Product Information

T lymphocytes are immune cells that respond to foreign antigens by secreting cytokines (CD8+ Cytotoxic T cells) and coordinating the immune response (CD4+ Helper T cells).

This panel is designed for the investigation of T lymphocytes (CD3 positive) and for the further separation of the T lymphocyte population into Helper T Lymphocyte (CD4 positive) and Cytotoxic T Lymphocyte (CD8 positive) subpopulations. The PE channel is left open. 

Panel Components

Fluorochrome (ex/em)	Catalog No	Target	Clone	Isotype	Description	Size
Cardinal Red	CR-65109	CD45	HI30	Mouse IgG1k	Lymphocyte marker	100 tests, 5uL/test
(592 nm/611 nm)
CoraLite Plus 405	CL405-65151	CD3	UCHT1	Mouse IgG1k	T cell marker	100 tests, 5uL/test
(399 nm/422 nm)
CoraLite Plus 750	CL750-65197	CD19	4G7	Mouse IgG1k	B cell marker	100 tests, 5uL/test
(755 nm/780 nm)
CoraLite Plus 488	CL488-65134	CD4	OKT4	Mouse IgG2bk	Helper T cell marker	100 tests, 5uL/test
(493 nm/522 nm)
CoraLite Plus 647	CL647-65135	CD8a	OKT8	Moues IgG2ak	Cytotoxic T cell marker	100 tests, 5uL/test
(654 nm/674 nm)

Storage

Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 

Usage

Human T Cell Basics Panel Datasheet and User Guide

Flow-Cytometry-Cell-Surface-Staining-Protocol

Cited in Article as

PK30007, Human T Cell Basics Panel, Proteintech, IL, USA