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CoraLite® Plus 488 Anti-Human CD4 (OKT4)

CD4 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OKT4

Cat no : CL488-65134

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 488 Anti-Human CD4 (OKT4) (CL488-65134)

    FC experiment of human peripheral blood lymphocytes using CL488-65134

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and 5.00 ul CoraLite® Plus 488-conjugated Anti-Human CD4 (CL488-65134, Clone: OKT4). Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using CoraLite® Plus 488 Anti-Human CD4 (OKT4) (CL488-65134)

    FC experiment of human peripheral blood lymphocytes using CL488-65134

    1X10^6 human peripheral blood lymphocytes were surface stained with APC Anti-Human CD3 (APC-65151, Clone: UCHT1) and CoraLite® Plus 488-conjugated Mouse IgG2b isotype control antibody. Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

CL488-65134 targets CD4 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Human peripheral T cells
Full Name CD4 molecule
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
RRIDAB_2883196
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD4 antibody CL488-65134Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Acta Pharm Sin B

Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

Authors - Renming Fan
View Article