Human ST2 ELISA Kit
Cat no : KE00055
Synonyms
DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1
Validation Data Gallery
Product Information
KE00055 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ST2 ELISA kit is to be used to detect and quantify protein levels of endogenous ST2. The assay recognizes human ST2. An antibody specific for ST2 has been pre-coated onto the microwells. The ST2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ST2 is added to detect the captured ST2 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ST2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9173 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 91% | 82%-103% |
| Cell culture supernatants | 100% | 80%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,058.5 | 30.8 | 2.9 |
| 2 | 20 | 247.2 | 5.6 | 2.3 |
| 3 | 20 | 61.9 | 1.6 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,054.8 | 40.8 | 3.9 |
| 2 | 24 | 257.3 | 7.1 | 2.7 |
| 3 | 24 | 72.4 | 4.4 | 6.1 |
Background Information
Protein ST2, also known as IL1RL1, is a member of the interleukin 1 receptor family. The gene of ST2 encodes three splice variants: a soluble secreted form (sST2), a transmembrane receptor form (ST2L), and a variant form of ST2 (ST2V). Interleukin-33 (IL-33) has been identified as a functional ligand of ST2L. IL-33 exerts its cellular functions by binding a receptor complex composed of ST2L and IL-1R accessory protein (IL-1RAcP). The interaction of IL-33 and ST2L activates mitogen-activated protein kinases and several biochemical pathways. The end of these reactions is the activation of the inhibitor of nuclear factor-kB (NF-kB) kinase complex, triggering NF-kB activity. sST2 seems to act as a decoy-receptor for IL-33: it binds IL-33 thus subtracting such a molecule from the interaction with ST2L. sST2 is formed by many cells and sST2 level is increased as response to myocardial stress, as well as in inflammatory conditions, including allergic asthma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DER4, FIT 1, IL1RL1, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Mediators Inflamm Serum Soluble ST2 Correlated with Symptom Severity and Clinical Response of Sublingual Immunotherapy for House Dust Mite-Induced Allergic Rhinitis Patients. |