Human Progranulin/PCDGF ELISA Kit
Cat no : KE00260
Synonyms
GEP, GP88, granulin, Granulins, GRN, KE00260, PCDGF, PGRN, Proepithelin, Progranulin
Validation Data Gallery
Product Information
KE00260 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Progranulin/PCDGF ELISA kit is to be used to detect and quantify protein levels of endogenous Progranulin/PCDGF. The assay recognizes human Progranulin/PCDGF. An antibody specific for Progranulin/PCDGF has been pre-coated onto the microwells. The Progranulin/PCDGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Progranulin/PCDGF is added to detect the captured Progranulin/PCDGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Progranulin/PCDGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Saliva, Urine, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 6.25-400 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2896 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Saliva | 102% | 92%-123% |
| Human plamsa | 100% | 92%-110% |
| Cell culture supernatants | 115% | 100%-125% |
| Urine | 95% | 74%-124% |
| Human milk | 110% | 84%-127% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 57.29 | 1.61 | 2.8 |
| 2 | 20 | 102.27 | 4.25 | 4.2 |
| 3 | 20 | 189.93 | 7.59 | 4.0 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 56.16 | 2.84 | 5.1 |
| 2 | 24 | 96.77 | 5.2 | 5.4 |
| 3 | 24 | 182.62 | 13.12 | 7.2 |
Background Information
Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, was first identified as a 593-aa secreted glycoprotein involved in the regulation of cancer progression and wound healing. It is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin (PGRN) has been considered a growth factor-like molecule implicated in additional biological and pathological processes including early embryogenesis, inflammation, host defense, cartilage development and degradation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GEP, GP88, granulin, Granulins, GRN, KE00260, PCDGF, PGRN, Proepithelin, Progranulin |