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Human Monocyte Basics Panel

Host

Mouse

Reactivity

Human

Applications

FC

Conjugate

Various

Cat no : PK30010

Validation Data Gallery

Applications:
  • 1x10^6 Human PBMCs were stained with PK30010 Human Monocyte Basics Panel. CD3-/HLA-DR+ cells are gated. Parent population: monocytes. Cells were not fixed.

    1x10^6 Human PBMCs were stained with PK30010 Human Monocyte Basics Panel. CD3-/HLA-DR+ cells are gated. Parent population: monocytes. Cells were not fixed.

  • 1x10^6 Human PBMCs were stained with PK30010 Human Monocyte Basics Panel. CD14 and CD16 expression on CD3-/HLA-DR+ monocytes are shown. Cells were not fixed.

    1x10^6 Human PBMCs were stained with PK30010 Human Monocyte Basics Panel. CD14 and CD16 expression on CD3-/HLA-DR+ monocytes are shown. Cells were not fixed.

  • 1x10^6 Human PBMCs were stained with PK30010 Human Monocyte Basics Panel. CD64 expression on CD14bright/CD16- (left) and CD14dim/CD16+ (right) cells is shown. Parent population: CD3-/HLA-DR+ cells.

    1x10^6 Human PBMCs were stained with PK30010 Human Monocyte Basics Panel. CD64 expression on CD14bright/CD16- (left) and CD14dim/CD16+ (right) cells is shown. Parent population: CD3-/HLA-DR+ cells.

Product Information

Monocytes are immune cells that monitor for signals of infection or tissue damage and respond through phagocytosis and antigen presentation or differentiation into macrophages or dendritic cells.

This panel is designed for the investigation of Monocytes (CD14+/HLA-DR+) and for the further separation of the Monocyte population into Classical, Intermediate, and non-Classical Monocyte subpopulations. The PE channel is left open. 


Panel Components

Fluorochrome (ex/em) Catalog No Target Clone Isotype Description
CoraLite Plus 647 CL647-65133             CD3 OKT3 Mouse IgG2ak Negative on monocytes
(654 nm/674 nm)
Atlantic Blue AB-65218   HLA-DR L243 Mouse IgG2ak Monocyte marker
(404 nm/458 nm)
CoraLite Plus 750 CL750-65246             CD14 26IC Mouse IgG2b Monocyte marker
(755 nm/780 nm)
Cardinal Red CR-65090             CD16 3G8 Mouse IgG1k Distinguishes Classical vs. non-Classical Monocytes
(592 nm/611 nm)
FITC Plus FITC-65253             CD64 10.1 Mouse IgG1k High expression on Classical Monocytes
(495 nm/524 nm)

Storage

Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.

Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 

Cited in Article as

PK30010, Human Monocyte Basics Panel, Proteintech, IL, USA