Recombinant Human MOG protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2878

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Validation Data Gallery

• 

  Purity of Recombinant Human MOG was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human MOG protein Gly30-Gly154 (Accession# Q16653-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 4340
• Accession: Q16653-1
• PredictedSize: 40.3 kDa
• SDS-PAGE: 40-50 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Myelin oligodendrocyte glycoprotein (MOG), which is exclusively expressed in oligodendrocytes, is a component of the outer surface of myelin in the central nervous system (CNS). Although a quantitatively minor component, MOG has strong antigenicity. In fact, MOG was initially identified as an immunodominant target for demyelinating autoantibodies in a guinea pig model of experimental autoimmune encephalomyelitis (EAE).

References:

1. Peschl P. et al. (2017) Front. Immunol. 8:529.
2. Litzenburger T. et al. (1998) J. Exp. Med. 188:169–180.
3. Pollinger B. et al. (2009) J. Exp. Med. 206:1303–1316.