AuthentiKine™ Human LIF ELISA Kit
Cat no : KE00208
Synonyms
CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI
Validation Data Gallery
Product Information
KE00208 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LIF ELISA kit is to be used to detect and quantify protein levels of endogenous LIF. The assay recognizes human LIF. An antibody specific for LIF has been pre-coated onto the microwells. The LIF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for LIF is added to detect the captured LIF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human LIF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture superntants |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3976 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 80% | 75%-85% |
| Cell culture superntants | 85% | 76%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 171.7 | 7.2 | 4.2 |
| 2 | 20 | 332.8 | 14.3 | 4.3 |
| 3 | 20 | 711.2 | 38.5 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 160.8 | 5.0 | 3.1 |
| 2 | 24 | 329.5 | 11.8 | 3.6 |
| 3 | 24 | 696.0 | 29.4 | 4.2 |
Background Information
Leukemia inhibitory factor (LIF) is a multi-functional cytokine of the interleukin-6 (IL-6) superfamily. It utilises a receptor that consists of the LIF receptor β and gp130 and this receptor complex is also used by ciliary neurotrophic growth factor (CNTF), oncostatin M, cardiotrophin1 (CT1) and cardiotrophin-like cytokine (CLC). Functionally, LIF has been implicated in a many physiological processes including the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CDF, D factor, DIA, Emfilermin, HILDA, Leukemia inhibitory factor, LIF, Melanoma derived LPL inhibitor, MLPLI |