Human IgG4 Monoclonal antibody, PBS Only

Human IgG4 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A2

Cat no : 66408-1-PBS

Synonyms

Human IgG4, IgG4, IGHG4

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Validation Data Gallery

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  WB analysis of human plasma using 66408-1-Ig (same clone as 66408-1-PBS)

  human plasma (0.4 nL) were subjected to SDS PAGE followed by western blot with 66408-1-Ig (human IgG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 66408-1-Ig (same clone as 66408-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66408-1-Ig (same clone as 66408-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66408-1-Ig (IGHG4 Antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66408-1-Ig (same clone as 66408-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66408-1-Ig (Human IgG4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 66408-1-Ig (same clone as 66408-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG4 antibody (66408-1-Ig, Clone: 1A1A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66408-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66408-1-PBS targets Human IgG4 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: immunoglobulin heavy constant gamma 4 (G4m marker)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: AL928742
• Gene Symbol: IGHG4
• Gene ID (NCBI): 3503
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4.