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Human IgG lambda chain Monoclonal antibody, PBS Only

Human IgG lambda chain Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D12B5

Cat no : 67762-1-PBS

Synonyms

Human IgG lambda chain, IGLC, IGLC1

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Human IgG lambda chain Monoclonal antibody (67762-1-Ig)

    WB analysis of human plasma using 67762-1-Ig (same clone as 67762-1-PBS)

    80 nL human plasma were subjected to SDS PAGE followed by western blot with 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG lambda chain Monoclonal antibody (67762-1-Ig)

    IHC staining of human tonsillitis using 67762-1-Ig (same clone as 67762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Human IgG lambda chain Monoclonal antibody (67762-1-Ig)

    IHC staining of human tonsillitis using 67762-1-Ig (same clone as 67762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using Human IgG lambda chain Monoclonal antibody (67762-1-Ig)

    IF Staining of human appendicitis using 67762-1-Ig (same clone as 67762-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG lambda chain antibody (67762-1-Ig, Clone: 1D12B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67762-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67762-1-PBS targets Human IgG lambda chain in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Human IgG lambda chain fusion protein Ag14783
Full Name immunoglobulin lambda constant 1 (Mcg marker)
Calculated Molecular Weight 233 aa, 25 kDa
Observed Molecular Weight~25 kDa
GenBank Accession NumberBC007782
Gene Symbol IGLC1
Gene ID (NCBI) 3537
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

This antibody detects Lambda chain of human IgG.