AuthentiKine™ Human IL-6 ELISA Kit
Cat no : KE00139
Synonyms
B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6
Validation Data Gallery
Product Information
KE00139 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous IL6. The assay recognizes human IL6. An antibody specific for IL6 has been pre-coated onto the microwells. The IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL6 is added to detect the captured IL6 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3569 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 81%-103% |
| Cell culture supernatants | 98% | 86%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 270.7 | 9.4 | 3.5 |
| 2 | 20 | 64.5 | 3.6 | 5.6 |
| 3 | 20 | 14.9 | 0.6 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 288.4 | 16.5 | 5.7 |
| 2 | 24 | 63.3 | 2.0 | 3.2 |
| 3 | 24 | 14.8 | 0.5 | 3.5 |
Background Information
Interleukin-6 (IL6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6 |
Publications
| Species | Sample Type | Title |
|---|---|---|
BMC Med HBeAg mediates inflammatory functions of macrophages by TLR2 contributing to hepatic fibrosis. | ||
Oncogenesis Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer. | ||
Nanotoxicology Role of inflammation in the malignant transformation of pleural mesothelial cells induced by multi-walled carbon nanotubes. | ||
Emerg Microbes Infect Serological investigation of asymptomatic cases of SARS-CoV-2 infection reveals weak and declining antibody responses. | ||
Front Cell Dev Biol miR-7-5p Affects Brain Edema After Intracerebral Hemorrhage and Its Possible Mechanism. | ||
Front Immunol Circulating Type I Interferon Levels in the Early Phase of COVID-19 Are Associated With the Development of Respiratory Failure. |