AuthentiKine™ Human IL-28A ELISA Kit
Cat no : KE00237
Synonyms
Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, Interferon lambda 2, Interleukin 28A, ZCYTO20
Validation Data Gallery
Product Information
KE00237 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-28A ELISA kit is to be used to detect and quantify protein levels of endogenous IL-28A. The assay recognizes human IL-28A and IL-28B. An antibody specific for IL-28A has been pre-coated onto the microwells. The IL-28A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-28A is added to detect the captured IL-28A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-28A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.12 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 282616 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 80% | 72%-89% |
| Cell culture supernatants | 97% | 88%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 440.6 | 11.5 | 2.6 |
| 2 | 20 | 102.2 | 3.1 | 3.1 |
| 3 | 20 | 23.8 | 0.4 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 428.9 | 10.7 | 2.5 |
| 2 | 24 | 97.7 | 1.6 | 1.6 |
| 3 | 24 | 24.1 | 0.9 | 3.9 |
Background Information
IL28A, also known as IFNL2, is a cytokine distantly related to type I interferons and the IL-10 family. IL-28A, IL-28B and IL-29 are three closely related cytokines classified as type III IFNs, which share many of the biological effects of type I IFNs but may have fewer side effects due to a more selective receptor distribution. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). IL28A is believed to play a significant role in the antiviral immune defense in the intestinal epithelium.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, Interferon lambda 2, Interleukin 28A, ZCYTO20 |