AuthentiKine™ Human IFN-gamma ELISA Kit


0.4 pg/mL


7.8-500 pg/mL



Cat no : KE00146


IFG, IFI, IFN gamma, IFNG, IFN-gamma, Immune interferon, Interferon gamma, interferon, gamma

Product Information

KE00146 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFN-gamma ELISA kit is to be used to detect and quantify protein levels of endogenous IFN-gamma. The assay recognizes human IFN-gamma. An antibody specific for IFN-gamma has been pre-coated onto the microwells. The IFN-gamma protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IFN-gamma is added to detect the captured IFN-gamma protein. For signal development, horseradish peroxidase (HRP)-conjugated is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name AuthentiKine™ Human IFN-gamma ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture superntants
Assay type Sandwich
Sensitivity 0.4 pg/mL
Range 7.8-500 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3458


Sample TypeAverageRange
Human plasma96%84%-107%
Cell culture superntants95%89%-107%


Samplenmean ( pg/mL)SDCV%


Samplenmean ( pg/mL)SDCV%

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.


Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms IFG, IFI, IFN gamma, IFNG, IFN-gamma, Immune interferon, Interferon gamma, interferon, gamma


SpeciesSample TypeTitle
human,mouseserum,cell culture supernatants

Front Immunol

Co-Expression of miR155 or LSD1 shRNA Increases the Anti-Tumor Functions of CD19 CAR-T Cells.

Authors - Jing Zhang
humancell culture supernatants

Eur J Immunol

Tissue-resident CD69+ CXCR6+ Natural Killer cells with exhausted phenotype accumulate in human non-small cell lung cancer

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Novel BCMA-OR-CD38 tandem-dual chimeric antigen receptor T cells robustly control multiple myeloma.

Authors - Yaru Feng
humancell culture supernatants

J Transl Med

Scoring model based on the signature of non-m6A-related neoantigen-coding lncRNAs assists in immune microenvironment analysis and TCR-neoantigen pair selection in gliomas

Authors - Wenbo Zhao
humancell culture supernatants

Sci Rep

Paeoniflorin drives the immunomodulatory effects of mesenchymal stem cells by regulating Th1/Th2 cytokines in oral lichen planus

Authors - Zhihui Zhang
humancell culture supernatants


Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic Fe(3)O(4) nanoparticles for magnetic targeting immunotherapy of solid tumors

Authors - Songbo Zhao