Human HMGB1 ELISA Kit
Cat no : KE00343
Synonyms
DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1
Validation Data Gallery
Product Information
KE00343 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HMGB1 ELISA kit is to be used to detect and quantify protein levels of endogenous human HMGB1. The assay recognizes human HMGB1. An antibody specific for human HMGB1 has been pre-coated onto the microwells. The human HMGB1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HMGB1 is added to detect the captured human HMGB1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HMGB1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum， Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3146 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 75%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.78 | 0.47 | 9.92 |
| 2 | 20 | 1.18 | 0.06 | 4.80 |
| 3 | 20 | 0.50 | 0.03 | 5.96 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 7.50 | 0.72 | 9.54 |
| 2 | 24 | 1.70 | 0.14 | 8.28 |
| 3 | 24 | 0.60 | 0.04 | 7.51 |
Background Information
The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1 |