AuthentiKine™ Human HGF ELISA Kit
Cat no : KE00168
Synonyms
F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF
Validation Data Gallery
Product Information
KE00168 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HGF ELISA kit is to be used to detect and quantify protein levels of endogenous HGF. The assay recognizes human HGF. An antibody specific for HGF has been pre-coated onto the microwells. The HGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, the horseradish peroxidase (HRP)-conjugated detection antibody specific for HGF is added to detect the captured HGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human HGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.26 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3082 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 91% | 73%-112% |
| Human plasma | 91% | 73%-112% |
| Cell culture supernatants | 117% | 93%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.7 | 0.30 | 6.4 |
| 2 | 20 | 9.3 | 0.65 | 7.0 |
| 3 | 20 | 18.3 | 0.92 | 5.0 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.1 | 0.42 | 8.4 |
| 2 | 24 | 10.5 | 0.96 | 9.2 |
| 3 | 24 | 20.9 | 19.3 | 9.2 |
Background Information
Hepatocyte growth factor (HGF) is the most potent mitogen of mature hepatocytes in primary culture. HGF is derived from a biologically inactive single chain precursor of 728 amino acids (pro-HGF) localized mostly on the cell surface and in the extracellular matrix. The mature form produced following proteolytic cleavage is composed of a 69-kDa alpha-subunit (containing four kringle domains) and the 34 kDa beta-subunit, similar to the catalytic domain of serine proteases, but with amino acid substitutions in the active site. HGF is a pleiotropic cytokine which exerts a variety of effects on several cells, being involved in the regulation of many biological processes, such as inflammation, tissue repair, morphogenesis, angiogenesis, tumour propagation, immunomodulation of viral infections and cardio-metabolic activities.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF |
Publications
| Species | Sample Type | Title |
|---|---|---|
QJM Predicting survival and immune microenvironment in colorectal cancer: a STAT signaling-related signature. | ||
Drug Des Devel Ther Network Pharmacology Analysis and Machine-Learning Models Confirmed the Ability of YiShen HuoXue Decoction to Alleviate Renal Fibrosis by Inhibiting Pyroptosis | ||
Int J Mol Sci Transplantation of Chemical Compound-Induced Cells from Human Fibroblasts Improves Locomotor Recovery in a Spinal Cord Injury Rat Model | ||
Sci Rep Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels |