Human GOLM1 ELISA Kit
Cat no : KE00111
Synonyms
bA379P1.3, C9orf155, FLJ22634, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, PSEC0257, UNQ686/PRO1326
Validation Data Gallery
Product Information
KE00111 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GOLM1 ELISA kit is to be used to detect and quantify protein levels of endogenous GOLM1. The assay recognizes human GOLM1. An antibody specific for GOLM1 has been pre-coated onto the microwells. The GOLM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for GOLM1 is added to detect the captured GOLM1 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GOLM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156 - 10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51280 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 102% | 78%-118% |
| Human plasma | 95% | 77%-114% |
| Human serum | 104% | 86%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.04 | 0.18 | 4.5 |
| 2 | 20 | 1.00 | 0.04 | 3.6 |
| 3 | 20 | 0.25 | 0.01 | 2.5 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3.91 | 0.19 | 4.8 |
| 2 | 24 | 0.96 | 0.04 | 3.8 |
| 3 | 24 | 0.23 | 0.01 | 2.7 |
Background Information
GOLM1 (also known as GP73 or GOLPH2) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLM1 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLM1 could be a promising serum marker for hepatocellular carcinoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | bA379P1.3, C9orf155, FLJ22634, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, PSEC0257, UNQ686/PRO1326 |