Human FABP2 ELISA Kit
Cat no : KE00263
Synonyms
FABP2, FABPI, Fatty acid binding protein 2, I FABP
Validation Data Gallery
Product Information
KE00263 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FABP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human FABP2. An antibody specific for FABP2 has been pre-coated onto the microwells. The FABP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for FABP2 is added to detect the captured FABP2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FABP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.8 pg/mL |
| Range | 156.3-10000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2169 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 105% | 90%-123% |
| Cell Lysate | 100% | 89%-116% |
| Cell culture supernant | 100% | 83%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4,105.7 | 213.2 | 5.2 |
| 2 | 20 | 990.7 | 27.3 | 2.8 |
| 3 | 20 | 252.6 | 8.9 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4,412.0 | 271.2 | 6.1 |
| 2 | 24 | 1022.1 | 61.0 | 5.9 |
| 3 | 24 | 251.9 | 8.7 | 3.5 |
Background Information
FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FABP2, FABPI, Fatty acid binding protein 2, I FABP |