Human CYCS ELISA Kit
Cat no : KE00079
Synonyms
CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4
Validation Data Gallery
Product Information
KE00079 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CYCS ELISA kit is to be used to detect and quantify protein levels of endogenous CYCS. The assay recognizes human CYCS. An antibody specific for CYCS has been pre-coated onto the microwells. The CYCS protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for CYCS is added to detect the captured CYCS protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CYCS ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54205 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 96% | 74%-126% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 545.4 | 21.6 | 4.0 |
| 2 | 20 | 1,398.4 | 51.0 | 3.6 |
| 3 | 20 | 2,467.2 | 119.3 | 4.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 513.0 | 45.9 | 9.0 |
| 2 | 24 | 1,370.1 | 90.9 | 6.6 |
| 3 | 24 | 2,503.1 | 97.8 | 3.9 |
Background Information
CYCS, Cytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, cytochrome c can be released from mitochondria into cytoplasm, resulting in caspase-3 activation and apoptosis. Measurement of cytochrome c release from the mitochondria is useful for detection of the onset of apoptosis in cells. In addition, cytochrome c can also leave cells and be detectable in extra-cellular medium of apoptotic cells and serum of cancer patients. The level of serum cytochrome c may serve as a prognostic maker during cancer therapy. This kit is designed to quantify the level of cytochrome c protein.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CYC, CYCS, Cytochrome c, cytochrome c, somatic, HCS, THC4 |