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Recombinant Human CD98/SLC3A2 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1948

Validation Data Gallery

  • Purity of Recombinant Human CD98 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD98 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD98 protein Arg206-Ala630 (Accession# P08195-1) with a rabbit IgG Fc tag at the N-terminus.
GeneID 6520
Accession P08195-1
PredictedSize 74.0 kDa
SDS-PAGE 70-95 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter) and an 85-kDa glycosylated heavy chain (CD98hc) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).

References:

1. Merlin D. et al. (2001). J Biol Chem. 276(42):39282-39289.
2. Fei F. et al. (2014). Ann Surg Oncol. 21(13):4359-4368.
3. Yan Y. et al. (2008). Biochim Biophys Acta. 1780(10):1087-1092.