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Human CD8 Memory T Cell Isolation Kit

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : KMS307

Validation Data Gallery

  • Following cell separation, cell suspension was stained with PB450-CD3 (UCHT1), FITC-CD8 (OKT8), PE-CD45RO (UCHL1) and CL647-CD45RA (HI100) antibodies. All viable cells are gated in the analysis. Left upper panel: CD3+CD8+ cells before selection; right upper panel: CD45RO+ cells within CD8+ cells. Left lower panel: CD3+CD8+ cells after selection; right lower panel: CD45RO+ cells within CD8+ cells. Human CD8 memory T cell isolation kit is tested using PBMC from three donors.

    Following cell separation, cell suspension was stained with PB450-CD3 (UCHT1), FITC-CD8 (OKT8), PE-CD45RO (UCHL1) and CL647-CD45RA (HI100) antibodies. All viable cells are gated in the analysis. Left upper panel: CD3+CD8+ cells before selection; right upper panel: CD45RO+ cells within CD8+ cells. Left lower panel: CD3+CD8+ cells after selection; right lower panel: CD45RO+ cells within CD8+ cells. Human CD8 memory T cell isolation kit is tested using PBMC from three donors.

Product Information

Memory T cells are antigen-exposed T lymphocytes which would mediate fast and robust response upon repeat encounter with antigen.  Human CD8 Memory T Cell Isolation kit is designed to isolate untouched CD8+ memory T lymphocytes which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD8+ memory cells will be retained in the tube by magnetic separator. The untouched CD8+ T memory cells are collected in supernatant. Any downstream application can be performed on CD8+ memory cells after separation, such as Flow Cytometry and in vitro culture. 

Components

KMS307-10:  

  ·MS307A-10: 300 µL Human CD8 Isolation Biotin Antibody Cocktail (CD4, CD19, CD14, CD56, CD45RA, CD20, CD11b, CD16, CD36, CD235a, CD123, TCR γδ)

  ·MS307B-10: 300µL streptavidin magnetic beads  

KMS307-100:  

  ·MS307A-100: 3mL Human CD8 Isolation Biotin Antibody Cocktail (CD4, CD19, CD14, CD56, CD45RA, CD20, 11b, CD16, CD36, CD235a, CD123, TCR γδ)

  ·MS307B-100: 3mL streptavidin magnetic beads 


Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Reactivity

Human

Beads diameter

2.7 µm

Recommend usage

30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1x107 PBMCs 


Cited in Article as

KMS307, Human CD8 Memory T Cell Isolation Kit, Proteintech, IL, USA

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Matthias (Verified Customer) (07-04-2024)

The isolation worked quite well, with an easy handling and quick processing. Cell yield was as expected with a puirty of 80-85% of the expected cell type.

  • Applications: Flow Cytometry,Cell culture
  • Cell Tissue Type: primary Leukocytes