Human CD71 ELISA Kit
Cat no : KE00081
Synonyms
CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR
Validation Data Gallery
Product Information
KE00081 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD71 ELISA kit is to be used to detect and quantify protein levels of endogenous CD71. The assay recognizes human CD71. A polyclonal antibody specific for CD71 has been pre-coated onto the microwells. The CD71 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD71 is added to detect the captured CD71 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human CD71 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.2 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7037 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 92% | 83%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein which mediates cellular uptake of iron from transferrin, a plasma protein which transports iron in the circulation. CD71 is a homodimer of two identical transmembrane subunits linked by two disulfide bonds. CD71 is almost ubiquitously expressed, with highest expression levels on some cells and tissues, including immature erythroid cells, placental tissue, and rapidly dividing cells. Soluble CD71 present in the circulation is the truncated form of the cell surface receptor. The concentration of serum CD71 has been suggested to provide a sensitive measure of iron depletion.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR |