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Recombinant Human CD68 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2061

Validation Data Gallery

  • Purity of Recombinant Human CD68 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD68 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD68 protein Asn22-Ser319 (Accession# P34810-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 968
Accession P34810-1
PredictedSize 60.8 kDa
SDS-PAGE 80-110 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD68 is a 110 kDa transmembrane glycoprotein widely expressed in monocyte cell types, such as macrophages, microglia, and osteoclasts. It is located at the lysosome and plasma membrane and is essential in various physiological and pathological processes, including atherosclerosis formation, inflammation and auto-immunity, bone-resorbing promotion, and tumor progression. It participates in the cellular response to lipopolysaccharide/oxidized low-density lipoprotein particle stimulus and negative regulation of dendritic cell antigen processing and presentation.

References:

1. C L Holness, et al. (1993) Blood. 81(6):1607-1613.
2. Maria C de Beer, et al. (2003) J Lipid Res. 44(4):674-685.
3. Li Song, et al. (2011) J Lipid Res. 52(8):1542-1550.
4. Jason W Ashley, et al. (2011) PLoS One. 6(10):e25838.
5. Gunnar Steinert, et al. (2014) Cancer Res. 74(6):1694-1704.
6. Giuseppe Troiano, et al. (2019) Oral Oncol. 93:66-75.