Human CD3 magnetic beads

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : MS002

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Validation Data Gallery

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  Following depletion of CD3+ cells, supernatant cell suspension was stained with FITC-CD45(F10-89-4) and CL647-CD3(UCHT1) antibodies. Left panel: CD3+CD45+ cells before selection. Right panel: CD3+CD45+ cells after depletion. All viable cells are gated in the analysis. Human CD3 magnetic beads are tested using PBMC from three different donors.

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Product Information

CD3 is a multimeric protein associated with T cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction. 45%-70% human peripheral blood mononuclear cells (PBMC) express CD3. Human CD3 Magnetic Beads are used for isolation or depletion of human CD3 T lymphocytes from PBMC, whole blood, or other sample types. Following incubation with human CD3 antibody conjugated magnetic beads, the cell sample is placed on a magnet. CD3+ cells remain attached to magnetic beads after separation and can be used for further downstream applications, such as in cell expansion, but not suitable for flow cytometry analysis. CD3- cells remain in supernatant and could also be used for further applications.

Components

MS002-10: 200 µL 10 mg/mL Human CD3 Magnetic Beads

MS002-100: 2 x 1 mL 10 mg/mL Human CD3 Magnetic Beads

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide.

Reactivity

Human

Recommend usage

20 µL Human CD3 Magnetic Beads for 1*10⁷ cells

Beads Diameter

2.7 μm

Cited in Article as

MS002, Human CD3 magnetic beads, Proteintech, IL, USA