Human CD3 T Cell Isolation Kit

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

Cat no : KMS309

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Validation Data Gallery

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  Following cell separation, cell suspension was stained with PB450-CD3 (UCHT1) and FITC-CD45 (HI30) antibodies. All viable cells are gated in the analysis. Left upper panel: CD3+CD4+ cells before selection; right upper panel: CD3+CD4+ cells after selection. Human CD3 T cell isolation kit is tested using PBMC from three donors.

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Product Information

CD3 is a multimeric protein associated with T cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction. Human CD3 T Cell Isolation kit is designed to isolate untouched CD3 T cells which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non-CD3 cells will be retained in the tube by magnetic separator. The untouched CD3 T cells are collected in supernatant. Any downstream application can be performed on CD3 T cells after separation, such as Flow Cytometry and in vitro culture. 

Components

KMS309-10:  

  · MS309A-10: 300µL Human CD3 T Cell Isolation Biotin Antibody Cocktail (CD14, CD15, CD19, CD56, CD36, CD235a, CD123)

  · MS309B-10: 300µL streptavidin magnetic beads  

KMS309-100:  

  · MS309A-100: 3mL Human CD3 T Cell Isolation Biotin Antibody Cocktail (CD14, CD15, CD19, CD56, CD36, CD235a, CD123)

  · MS309B-100: 3mL streptavidin magnetic beads 

Storage

2-8°C

Storage buffer

PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide  

Reactivity

Human

Beads diameter

2.7 µm

Recommend usage

30µL biotin labeled antibody cocktail and 30µL streptavidin beads for 1x10⁷ cells 

Cited in Article as

KMS309, Human CD3 T Cell Isolation Kit, Proteintech, IL, USA