Human CD3 Magnetic Beads Kit

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Application

Cell Separation

Conjugate

Magnetic Beads

CloneNo.

OKT3

Publications

1

Cat no : KMS001

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Validation Data Gallery

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  Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and PE-CD3(UCHT1) antibodies. All viable cells are gated in the analysis. Left panel: CD3+CD45+ cells before selection. Middle panel: CD3+CD45+ cells after enrichment. Right panel: CD3+CD45+ cells after depletion. Human CD3 magnetic beads kit is tested using PBMC from three donors.

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Product Information

CD3 is a multimeric protein associated with T cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction. 45%-70% human peripheral blood mononuclear cells (PBMC) express CD3. Human CD3 Magnetic Beads Kit is used for isolation or depletion of human CD3 T lymphocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD3 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD3+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD3- cells remain in supernatant and can also be used for further applications.

Components

KMS001-10:

  · MS001-10: 100µL 10mg/mL streptavidin magnetic beads

  · MS65133-10: 100µL 0.1mg/mL Biotin-CD3 (clone: OKT3)

KMS001-100:

  · MS001-100: 1mL 10mg/mL streptavidin magnetic beads

  · MS65133-100: 1mL 0.1mg/mL Biotin-CD3 (clone: OKT3)

Storage

2-8°C

Storage buffer

Streptavidin beads: PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Biotin Antibody: PBS, pH7.4, 0.2% BSA and 0.09% Sodium Azide

Reactivity

Human

Recommend usage

10µL Biotin-CD3 antibody and 10µL streptavidin beads for 1*10⁷ cells

Cited in Article as

KMS001, Human CD3 Magnetic Beads Kit, Proteintech, IL, USA

Publications

Application	Title
	Immunol Med; C5a stimulation induces caspase-1 activation and mature IL-1β production in human peripheral blood mononuclear cells; Authors - Yuya Fujita